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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
上海远慕生物
- 规格:
100g
产品名称:Nutrient Broth 营养肉汤
品牌:BD
货号:YM-233000-100g
规格:100g
代理商:上海远慕生物
Nutrient Broth 营养肉汤实验内容:
1.称量→溶化→调pH→过滤→分装→加塞→包扎→灭菌→无菌检查;
2.干热灭菌:装入待灭菌物品→升温→恒温→降温→开箱取物;
3.高压蒸汽灭菌:加水→装物品→加盖→加热→排冷空气→加压→恒压→降压回零→排汽→取物→无菌检查;
4.过滤除菌:组装灭菌→连接→压滤→无菌检查→清洗灭菌;
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文献和实验.EukaryotesBy contrast to the simple broth cultures of E. coli, the nutrient requirements of even the simplest eukaryotic culture are complex. Refer to Table 12.1 for a comparison of the ingredients of Nutrient Broth and Minimum Essential Media (MEM), a typical
Alternate Sporulation Method for BY4743
3% Difco nutrient broth 3 gm 15 gm 1% Difco yeast extract 1 gm 5 gm 2% Bacto agar
Bacterial Transformation(细菌转化)
” can mean in excess of 109 colonies produced per %26micro;g of (purified) DNA. For subcloning and mutagenesis is normally sufficient, although “Library efficiency” bacteria may be useful if problems arise.Materials required Competent cells nutrient broth
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