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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
Pasteur, Paris, France Excerpted from Protein: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT cAMP measurements are obtained using an ELISA assay (Harlow
Use of the DiscoveRx Hithunter cAMPII Assay for Direct Measurement of cAMP in Gs and Gi GPCRs
and classical orthosteric antagonists. Several different homogenous assay systems can be used to monitor the signaling cascades of GPCRs. This chapter details the authors’ collective experience of using the DiscoverX Hit Hunter cAMPII kit from GE Healthcare
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