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- Sino Biological
- 50697-MNAE
- 2025年08月12日
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- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：EMAP-II/AIMP1蛋白, EMAP-II/AIMP1 protein
蛋白构建：A DNA sequence encoding the mouse SCYE1 (Q62386) (Ser145-Lys310) was expressed and purified.
表达宿主：E. coli
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant mouse SCYE1 consists of 166 amino acids and predicts a molecular mass of 18 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：Q62386
蛋白氨基酸序列：Ser145-Lys310
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验from their mothers ii. discard un-needed non-transgenic pups iii. house males and females separately f) when mice are 6 weeks old they may be mated (see 1. above) ES Cell-Mouse Chimera Breeding Based on our experience, we propose the following
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
Equilibration Buffer inro the column. Incubate at room temperature for 5 minutes. Spin at maximum speed (>13,000 x g) for 1 minute. 9. Apply 750 ul of mixture from step 7 to the HiBind® Mini column II (purple) pre-inserted in a 2 ml collection tube
Materials: 250 µL of fresh mouse blood in plastic tubes containing EDTA. RBC lysis buffer (388 mM NH4 Cl, 29.7 mM NaHCO3 , 25 µM Na2 EDTA) 20.75g. NH4 CL 2.5g NaHCO3 0.093g Na2 EDTA 1L H2
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