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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human GITR / TNFRSF18 Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥2980.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥21030.0 |
蛋白名称:Human GITR / TNFRSF18 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the human TNFRSF18 (Q9Y5U5-1) (Met1-Glu161) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 26
蛋白分子量:The recombinant human TNFRSF18/Fc is a disulfide-linked homodimer. The reduced monomer comprises 377 amino acids and has a predicted molecular mass of 41.5 kDa. The apparent molecular mass of the protein is approximately 96 and 45 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q9Y5U5-1
蛋白氨基酸序列:Met1-Glu161
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
, and analysis. A protocol is described for enhanced detection of low?affinity interactions by generating multivalent complexes using Fc?fusion bait proteins and protein A microbeads, along with a statistical method for hit scoring and identification
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
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