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100 µL
抗体种属:Canine
抗体靶点:CXCL13
抗体应用:ELISA
抗原货号:70057-D07E
抗原描述:Recombinant Canine BCA-1 / CXCL13 protein (Catalog#70057-D07E)
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A
抗体制备:Produced in rabbits immunized with purified, recombinant Canine BCA-1 / CXCL13 (Catalog#70057-D07E; XP_851619.3; Val23-Asn110). Total IgG was purified by Protein A affinity chromatography.
抗体特异性:Canine BCA-1 / CXCL13
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Bicinchoninic Acid (BCA) Protein Assay (Smith)
: 4 gm BCA in 100 ml distilled water. 3.Reagent C: 0.4 gm cupric sulfate (5 x hydrated)in 10 ml water. 4.Working solution: Mix 1 volume reagent C with 25 volumes reagent B,then add 26 volumes reagent A to the C/B mixture. Assay 1.Prepare samples
Quantitation of Protein:Bicinchoninic Acid (BCA) Method
【 Purpose 】 Master the BCA method and procedure to quantitate protein concentration 【 Principle 】 BCA method is a modification of Lowry method. The BCA technique offers manipulative
蛋白浓度不受绝大部分样品中的去污剂等化学物质的影响,可以兼容样品中高达5%的SDS,5%的Triton X-100,5%的Tween 20,60,80。 3. 在20-2000μg/ml浓度范围内有良好的线性关系。 4. 检测不同蛋白质分子的变异系数远小于考马斯亮蓝法蛋白定量。 5. 受螯合剂和略高浓度的还原剂的影响:EDTA小于10mM。DTT小于1mM巯基乙醇低于1mM BCA法和Commassie法各有优势,在不知道蛋白样本缓冲液成分的情况下可以两种方法配合使用,以消除定量
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