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- Sino Biological
- 51134-T16
- 2025年08月12日
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100 µL
抗体种属：Mouse
抗体靶点：ALPL
抗体应用：ELISA
抗原货号：51134-M08H
抗原描述：Recombinant Mouse Alkaline Phosphatase / ALPL protein (Catalog#51134-M08H)
抗体宿主：Rabbit
抗体Ig类型：Rabbit IgG
抗体纯化方法：Protein A & Antigen Affinity
抗体制备：Produced in rabbits immunized with purified, recombinant Mouse Alkaline Phosphatase / ALPL (Catalog#51134-M08H; BAH03518.1; Met1-Ser502). Alkaline Phosphatase / ALPL specific IgG was purified by Mouse Alkaline Phosphatase / ALPL affinity chromatography.
抗体特异性：Mouse Alkaline Phosphatase / ALPL
抗体保存：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Purification of acidic phosphatase from mustard seedlings
at which such phosphatases have optimal activity, we distinguish between acidic phosphatases (also called acid phosphatases) and alkaline phosphatases. The latter enzymes require divalent metal ions as cofactors and are common in animal tissues and bacteria. Acidic
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