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100 µL
抗体种属:Human
抗体靶点:MAPK12
抗体应用:WB,ELISA,IP
抗原货号:15676-H20B
抗原描述:Recombinant Human ERK3 / MAPK12 / P38-gamma Protein (Catalog#15676-H20B)
抗体宿主:Rabbit
抗体Ig类型:Rabbit IgG
抗体纯化方法:Protein A & Antigen Affinity
抗体制备:Produced in rabbits immunized with purified, recombinant Human ERK3 / MAPK12 / P38-gamma (rh ERK3 / MAPK12 / P38-gamma; Catalog#15676-H20B; P53778; Met1-Leu367). ERK3 / MAPK12 / P38-gamma specific IgG was purified by Human ERK3 / MAPK12 / P38-gamma affinity chromatography.
抗体特异性:Human ERK3 / MAPK12 / P38-gamma
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Thawing Cells - Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. - Add the 1ml
THAWING CELLS Remove a vial of Passage 2 Primary Mouse Embryo Fibroblasts (PMEF'S), at a concentration of 3 x 106 from liquid nitrogen and thaw quickly at 37oC. Add the 1ml of cell containing media to 9ml of warmed PMEF media
FEEDER PREPARATION BY GAMMA IRRADIATION
FEEDER PREPARATION BY GAMMA IRRADIATION All procedures should be carried out using sterile techniques at all times. Media for STOs/ SNL 76/7 cells is DMEM (high glucose, no pyruvate), 7% FBS and 1X GPS. 1. Gelatinize plates
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