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- Sino Biological
- 10602-RP03
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µL
抗体种属：Human
抗体靶点：TNF
抗体应用：WB,ELISA
抗原货号：10602-HNAE
抗原描述：Recombinant Human TNFα protein (Catalog#10602-HNAE)
抗体宿主：Rabbit
抗体Ig类型：Rabbit IgG
抗体纯化方法：Protein A
抗体标记：
抗体制备：Produced in rabbits immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; Catalog #10602-HNAE; Val 77-Leu 233; NP_000585.2). Total IgG was purified by Protein A affinity chromatography .
抗体特异性：Human TNFα
抗体保存：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Radioactive DNA Fragmentation Assay
DESCRIPTION of the method: The DNA Fragmentation Assay allows to determine the amount of DNA that is degraded upon treatment of cells with certain agents, e.g. with TNF-alpha or anti-Fas antibody (IPO-4).
Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
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