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20 µL/100 µL
| 规格: | 20 µL | 产品价格: | ¥600 |
|---|---|---|---|
| 规格: | 100 µL | 产品价格: | ¥1699 |
抗体种属:Human
抗体靶点:MMP9
抗体应用:ELISA
抗原货号:10327-H08H
抗原描述:Recombinant Human MMP-9 protein (Catalog#10327-H08H)
抗体宿主:Mouse
抗体Ig类型:Mouse IgG1
抗体纯化方法:Protein A
抗体制备:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human matrix metallopeptidase 9 (rhMMP9; Catalog#10327-H08H; Met1-Asp707; NP_004985.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性:Human MMP9 / CLG4B
抗体保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
methods that can accurately determine the genotypes and phenotypes of human complement components C4A and C4B. Specifically, they allow investigators to determine how many C4 genes are present in a diploid genome of a human subject and to quantify
我做的是大鼠肾脏 MMP-9 和 PAI-1 的免疫组化染色。MMP-9 为山羊抗鼠的,PAI-1 为兔抗鼠的,均为中山公司产品。选用中山公司相应的二抗试剂盒。反复做了好几批片子可结果总是不尽如人意,背景颜色比较深,难以判断阳性结果。 具体步骤如下: 1、石蜡包埋组织切片 4μm,用的是中山公司的多聚赖氨酸防脱片的玻片,捞片后置 60℃ 烤箱烤片 2-4 小时。 2、半月后,脱蜡水化 3、PBS 液冲洗,3 分钟×3; 4、切片置于 1%triton-x-100
A 或者protein G,或者是protein A/protein G组合。一般推荐小鼠单抗IgG2a,IgG2b,IgG3,兔,人,猪的多克隆抗体用protein A纯化,而小鼠单抗IgG1, 大鼠单抗,小鼠,大鼠,山羊的多克隆抗体则选择用protein G纯化。 具体实验步骤如下: 称取经CNBr活化了的sepharose 4B 0.5g, 溶于2mM的HCL溶液中,4摄氏度过夜,使其充分溶胀。 将溶胀好了的sepharose 4B预装于层析柱中,用10倍体积的2mM HCL溶液洗涤三次后用0.1 M
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