产品描述:
Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiological regulation of body weight, leptin has a variety of other physiological and pathological functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Rat Leptin produced in E. coli is a single non-glycosylated polypeptide chain containing 147 amino acids with a MW of 16,240 Da.
形 态 :
Each vial contains 1 mg protein lyophilized in 0.1% TFA. Reconstitute with 1 ml sterile deionized water for a final concentration of 1 mg/ml.
来 源 :
E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
别 名 :
leptin; obesity factor; obese protein; LEP
纯 度 :
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
分子量 :
16240 Da
应 用 :E, WB
rRLeptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependent stimulation of Human OB-R transfected mouse BaF3 indicator cells is 0.06 – 0.35 ng/ml.
保存条件 :
Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
产品链接 :http://www.abbiotec.com/proteins/rat-leptin