产品描述:
Macrophage colony stimulating factor (M-CSF) is one of the glycoproteins called colony-stimulating factors (CSFs). Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood: the granulocytes and the monocytes-macrophages. Mononuclear phagocyte colony-stimulating factor (M-CSF) is synthesized by mesenchymal cells. This cytokine stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. Some clinical investigations have shown autologous production of M-CSF various human cell lines in vitro and by tumors in vivo. Recombinant Human M-CSF produced in E. coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids with a MW of 36.8 kDa.
形 态 :
Each vial contains 10 ug protein lyophilized in 10 mM sodium phosphate pH8.0, 50 mM NaCl. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
来 源 :
E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
别 名 :
Macrophage colony-stimulating Factor 1; CSF-1; MCSF; M-CSF; Lanimostim; processed macrophage colony-stimulating factor 1; CSF1
纯 度 :
Purity > 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
分子量 :
36800 Da
应 用 :E, WB
Recombinant Human Macrophage Colony Stimulating Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse NFS-60 cells is <2.0 ng/ml.
保存条件 :
Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
产品链接 :http://www.abbiotec.com/proteins/human-mcsf