产品描述:
Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17RA receptor, its biological function is similar to that of IL-17A, i.e. inducing production of a variety of chemokines in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells. Recombinant human IL-17AF produced in E.coli is a heterodimeric non-glycosylated polypeptide comprised of one monomeric subunit each of human IL-17A and human IL-17F. The dimer consists of 271 amino acids, with an approximate molecular weight of 30.7 kDa. The recombinant protein corresponds to full-length IL-17A and IL-17F and has cytokine production activity on mouse cells.
形 态 :
Each vial contains 25 ug of lyophilized protein. Reconstitute with 0.25 ml sterile deionized water for a final concentration of 0.1 mg/ml.
来 源 :
E. coli. Endotoxin level as measured by LAL is <0.1 ng/ug or <1 EU/ug.
别 名 :
interleukin-17A; IL-17A; IL-17; Cytotoxic T-lymphocyte-associated antigen 8; IL17A; CTLA8; IL17; Interleukin-17F; IL-17F; Cytokine ML-1; IL17F
纯 度 :
Purity > 98% as determined by reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
分子量 :
30690 Da
应 用 :E, WB
The ED50 as determined by the dose-dependent production of IL-6 in cultured mouse NIH-3T3 fibroblast cells is typically 3-15 ng/ml.
保存条件 :
Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
产品链接 :http://www.abbiotec.com/proteins/human-il-17a/f