产品描述: Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system. IL-3 is important in several chronic inflammatory diseases. Recombinant Human IL-3 produced in E. coli is a single non-glycosylated polypeptide chain containing 133 amino acids with a MW of 15 kDa.
形 态 : Each vial contains 10 ug of lyophilized protein. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
来 源 : E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
别 名 : Interleukin-3; IL-3; multipotential colony-stimulating factor; hematopoietic growth factor; P-cell-stimulating factor; mast cell growth factor; MCGF
纯 度 : Purity > 95% as determined by analysis by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
分子量 : 15000 Da
应 用 :E, WB
Human IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 0.1 ng/ml.
保存条件 : Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.