Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
英文名:
EL4 Lysate
库存:
1000
规格:
0.1 mg
EL4细胞裂解物,EL4 Lysate(产品名称请以英文名称为准)【英文名称】:EL4 Lysate【货号】:1287【规格】:0.1 mg【保存条件】:Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.【简介】:EL4 lysate was prepared by homogenization in modified RIPA buffer (150mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mMethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1%Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed bycentrifugation. Protein concentration was determined with Bio-Radprotein assay. The EL4 lysate was boiled for 5 min in 1 x SDS samplebuffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate,0.01% bromophenol blue) containing 5% β-mercaptoethanol.EL4细胞裂解物,EL4 Lysate 公司竭诚为广大科研用户提供最全面,最优质,价格最具优势的产品,免费为您提供全程技术指导,解决您的后顾之忧。公司供应各种高品质科研试剂及各种规格包装定制,请联系我们.