人抗斑疹伤寒抗体(anti-typhus-Ab)ELISA检测试剂盒 规 格:48T/96T
价格:全国热线:400-991-0197,或咨询在线客服QQ,或者以邮件形式发到我司邮箱741653262@qq.com,齐一生物公司提供
使用范围:仅供科研使用,不得用于临床
本试剂仅供研究使用 目的:本试剂盒用于测定血清,血浆及相关液体样本中含量。
实验原理:
本试剂盒应用双抗体夹心法测定标本中水平。用纯化的本产品抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入本产品抗原,再与HRP标记的本产品抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的本产品呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中浓度。
试剂盒组成:
试剂盒组成 48孔配置 96孔配置 保存
说明书 1份 1份
封板膜 2片(48) 2片(96)
密封袋 1个 1个
酶标包被板 1×48 1×96 2-8℃保存
标准品:1800ng/L 0.5ml×1瓶 0.5ml×1瓶 2-8℃保存
标准品稀释液 1.5ml×1瓶 1.5ml×1瓶 2-8℃保存
酶标试剂 3 ml×1瓶 6 ml×1瓶 2-8℃保存
样品稀释液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
显色剂A液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
显色剂B液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
终止液 3ml×1瓶 6ml×1瓶 2-8℃保存
浓缩洗涤液 (20ml×20倍)×1瓶 (20ml×30倍)×1瓶 2-8℃保存
标本要求:
1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融
2.不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
操作步骤:
1.标准品的稀释与加样:在酶标包被板上设标准品孔10孔,在第一、第二孔中分别加标准品100μl,然后在第一、第二孔中加标准品稀释液50μl,混匀;然后从第一孔、第二孔中各取100μl分别加到第三孔和第四孔,再在第三、第四孔分别加标准品稀释液50μl,混匀;然后在第三孔和第四孔中先各取50μl弃掉,再各取50μl分别加到第五、第六孔中,再在第五、第六孔中分别加标准品稀释液50ul,混匀;混匀后从第五、第六孔中各取50μl分别加到第七、第八孔中,再在第七、第八孔中分别加标准品稀释液50μl,混匀后从第七、第八孔中分别取50μl加到第九、第十孔中,再在第九第十孔分别加标准品稀释液50μl,混匀后从第九第十孔中各取50μl弃掉。(稀释后各孔加样量都为50μl,浓度分别为1200 ng/L,800 ng/L ,400 ng/L,200ng/L, 100 ng/L)。
2.加样:分别设空白孔(空白对照孔不加样品及酶标试剂,其余各步操作相同)、待测样品孔。在酶标包被板上待测样品孔中先加样品稀释液40μl,然后再加待测样品10μl(样品最终稀释度为5倍)。加样将样品加于酶标板孔底部,尽量不触及孔壁,轻轻晃动混匀。
3.温育:用封板膜封板后置37℃温育30分钟。
4.配液:将30(48T的20倍)倍浓缩洗涤液用蒸馏水30(48T的20倍)倍稀释后备用。
5.洗涤:小心揭掉封板膜,弃去液体,甩干,每孔加满洗涤液,静置30秒后弃去,如此重复5次,拍干。
6.加酶:每孔加入酶标试剂50μl,空白孔除外。
7.温育:操作同3。
8.洗涤:操作同5。
9.显色:每孔先加入显色剂A50μl,再加入显色剂B50μl,轻轻震荡混匀,37℃避光显色15分钟.
10.终止:每孔加终止液50μl,终止反应(此时蓝色立转黄色)。
11.测定:以空白空调零,450nm波长依序测量各孔的吸光度(OD值)。 测定应在加终止液后15分钟以内进行。
人抗斑疹伤寒抗体(anti-typhus-Ab)ELISA检测试剂盒其它产品
Human anti-alpha-Fodrin IgG/IgA,α-Fodrin IgG/IgA ELISA试剂盒
human single stranded DNA Antibody,ssDNA ELISA试剂盒
Human histone H2A-H2B-DNA autoantibody ELISA试剂盒
Human anti-double stranded DNA,dsDNA ELISA试剂盒
human anti-nucleosome antibody IgG,AnuA-IgG ELISA试剂盒
Human anti-scl70 antibody,SCL70/topoⅠELISA试剂盒
Human SS-B/La Antibody,ELISA试剂盒
Human Pemphigus Foliaceus,PF ELISA试剂盒
Human β2-Glycoprotein 1 antibody IgA,β2-GP1 Ab IgA ELISA试剂盒
Human β2-Glycoprotein 1 Antibody IgM,β2-GP1 Ab IgM ELISA试剂盒
Human β2-Glycoprotein 1 Antibody IgG,β2-GP1 Ab IgG ELISA试剂盒
Human anti-cardiolipin antibody IgA,ACA-IgA ELISA试剂盒
Human anti-cardiolipin antibody IgM,ACA-IgM ELISA试剂盒
Human anti-cardiolipin antibody IgG,ACA-IgG ELISA试剂盒
Human anti-golgi apparatus antibody,AGAA ELISA试剂盒
Human Chlamydia trachomatis,CT ELISA试剂盒
human S100 calcium binding protein A9/calgranulin B,S100A9 ELISA试剂盒
human S100 calcium binding protein A8/calgranulin A,S100A8 ELISA试剂盒
human proviral integration site 1,Pim1 ELISA试剂盒
Human Pseudomonas Exotoxin A,PEA ELISA试剂盒
Human Influenza viruses A,FLU A ELISA试剂盒
Human hepatitis B virus surface antigen,HBsAg ELISA试剂盒
Human anti-hepatitis B virus core antibody,HBcAb ELISA试剂盒
Human anti-hepatitis B virus core IgM,HBc-IgM antibody ELISA试剂盒
Human anti-hepatitis B virus surface antibody,HBsAb ELISA试剂盒
Human anti-hepatitis B virus e antibody,HBeAb ELISA试剂盒
Human anti-epidemic hemorrhagic fever virus IgM antibody,EHF IgM ELISA试剂盒
Human anti-rabies virus antibody, anti-RV ELISA试剂盒
Human anti-hepatitis A virus IgM antibody,anti-HAV ELISA试剂盒
Human anti-hepatitis A virus IgG antibody,anti-HAV ELISA试剂盒
Human anti-parainfluenza virus IgM antibody, anti-PIV IgM ELISA试剂盒
Human anti-hepatitis D virus antibody,anti-HDV ELISA试剂盒
Human anti-typhus antibody ELISA试剂盒
Human listeriolysin O,LLO ELISA试剂盒
Human hepatitis B virus X Ag,HBxAg ELISA试剂盒
Human adeno-associated virus,AAV ELISA试剂盒
Human hepatitis B virus X interacting protein,HBXIP ELISA试剂盒
Human Salmonella typhi antigen,St-Ag ELISA试剂盒
Human T-lymphotropicvirusⅠ,HTLV-ⅠELISA试剂盒
Human morbillivirus,MV ELISA试剂盒
Human epidemic parotitis,EP ELISA试剂盒
Human anti-epidemic hemorrhagic fever virus IgG antibody,EHF IgG ELISA试剂盒
Human Entamoeba histolytica Antigen ELISA试剂盒
Human Lyme IgG ELISA试剂盒
Human Campylobacter Jejuni PEB1 ELISA试剂盒
Human chlamydial protease-like activity factor,CPAF ELISA试剂盒
Human Poliomyelitis Virus,PV-IgG ELISA试剂盒
Human Toxoplasma Gondii,T.gondii ELISA试剂盒
Human Poliomyelitis Virus,PV-IgG ELISA试剂盒
Human Bullous Pemphigoid,BP ELISA试剂盒
Human Enterovirus ELISA试剂盒
Human echinococcus antibody IgG ELISA试剂盒
Human Pertussiu Toxin,PT ELISA试剂盒
Human Candida Albicans,C.albicans ELISA试剂盒
Human Coronaviruses IgG ELISA试剂盒
Human Coronaviruses IgG ELISA试剂盒
Human heat-labile enterotoxin B subunit,LTB ELISA试剂盒
Human Chlamydia trachomatis,CT ELISA试剂盒
human schistosoma ELISA试剂盒
Human Rotavirus antigen,RV Ag ELISA试剂盒
Human Legionella pneumophila antigen,LP Ag ELISA试剂盒
human colicin ELISA试剂盒
Human Adenovirus Antigen,ADV-Ag ELISA试剂盒
Human Trichinella antibody ELISA试剂盒
Human Tetanus Antibody ELISA试剂盒
Human Lebtospira IgM ELISA试剂盒
Human Leptospira IgG,Lep IgG ELISA试剂盒
Human Amebiasis ELISA试剂盒
Human Diphtheria antibody ELISA试剂盒
Human Cysticercosis antybody,CYT Ab ELISA试剂盒
Human Brucella Antibody IgG,Brucella Ab IgG ELISA试剂盒
Human Varicella zoster virus IgM,VZV IgM ELISA试剂盒
Human Varicella zoster virus IgG,VZV-IgG ELISA试剂盒
Human Legionella antibody IgA,LP Ab-IgA ELISA试剂盒
Human Legionella antibody IgM,LP Ab-IgM ELISA试剂盒
Human Legionella antibody IgG,LP Ab-IgG ELISA试剂盒
Human paramyxovirus parotitis IgM ELISA试剂盒
Human paramyxovirus parotitis IgG ELISA试剂盒
Human epstein-barr virus IgM,EBv IgM ELISA试剂盒
Human epstein-barr virus IgA,EBv IgA ELISA试剂盒
Human epstein-barr virus IgG,EBv IgG ELISA试剂盒
Human Leishimaria antibody ELISA试剂盒
Human dengue fever antibody,DF ELISA试剂盒
Human Influenza viruses IgG,FLU ELISA试剂盒
Human Adenovirus IgM,ADV-IgM ELISA试剂盒
Human Adenovirus IgG,ADV-IgG ELISA试剂盒
Human Rotavirus,RV IgM ELISA试剂盒
human EchoVirus IgM,ECHO IgM ELISA试剂盒
Human anti-respiratory syncytial virus antibody,RSV ELISA试剂盒
Human measlesvirus IgM,MV IgM ELISA试剂盒
Human Coxsackie virus IgM,Cox V-IgM ELISA试剂盒
Human Coxsackie virus IgG,Cox V-IgG ELISA试剂盒
Human tuberculosis antibody IgG,TB-Ab IgG ELISA试剂盒
Human cicatricial Pemphigoid,CP ELISA试剂盒
human hepatitis B virus,HBV preS2Ab ELISA试剂盒
human hepatitis B virus,HBV preS2Ag ELISA试剂盒
Human Hepatitis G virus IgG,HGV-IgG ELISA试剂盒
human transfusion transmitted virus,TTV ELISA试剂盒
Human Hepatitis D virus IgM,HDV IgM ELISA试剂盒
human hepatitis E virus IgG,HEV-IgG ELISA试剂盒
Human Hepatitis D virus IgM,HDV IgM ELISA试剂盒
human Hepatitis D virus IgG,HDV IgG ELISA试剂盒
Human Hepatitis C virus IgM,HCV-IgM ELISA试剂盒
Human Hepatitis C virus IgG,HCV-IgG ELISA试剂盒
Human glucoprotein 130,gp130 ELISA试剂盒
Human corticotropin-releasing factor,CRF
Human Desmopressin/1-desamino-8-D-arginine vasopressin,dDAVP ELISA试剂盒
Human muscarinic acetylcholine receptor M3,M-AChR M3 ELISA试剂盒
Human Testoterone recepter,TR ELISA试剂盒
Human muscarinic acetylcholine receptor,M-AChR ELISA试剂盒
human glycoprotein 49A,Gp49a ELISA试剂盒
human inhibin beta-B,Inhbb ELISA试剂盒
Human Orexin receptor,OXR ELISA试剂盒
Human growth hormone releasing peptide-Ghrelin,GHRP-Ghrelin ELISA试剂盒
Human calmodulin,CAM ELISA试剂盒
Human true insulin,TI ELISA试剂盒