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- 详细信息
- 文献和实验
- 技术资料
- 库存：
5663
- 供应商：
齐一生物科技（上海）有限公司
- 检测范围：
详细请见说明书
- 检测方法：
ELISA酶联免疫法
- 应用：
科研检测
- 标记物：
见说明
- 样本：
血清，血浆，尿液，胸腹水，脑脊液，细胞培养上清，组织匀浆等
- 规格：
48T/96T
人抗红细胞抗体（RBC）ELISA试剂盒齐一生物科技（上海）有限公司产品已被广泛应用于化学、化工、生命科学的基础研究和开发应用、制药、疾病诊断与控制、人口与健康、生物技术等诸多领域.客户遍布国内各大学、研究所、医院、卫生防疫、商品检验检疫、制药公司、生物技术公司和食品工业等单位.齐一生物科技（上海）有限公司客服热线：021-60348496.Web:www.qiyibio.com
规 格：96T/48T
检测标本：血清，血浆，尿液，胸腹水，脑脊液，细胞培养上清，组织匀浆等
检测的原理：试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）
产品的用途：仅供科研课题使用
试剂盒保存：2-8℃。
有效期：6个月
运输方法：泡沫箱配生物冰袋运输
人抗红细胞抗体（RBC）ELISA试剂盒价格及详细资料：电议,或咨询在线客服QQ741653262,或者以邮件形式发到我司邮箱qysw@qiyibio.com .齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大科研单位一致肯定和认同。大品牌保证，价格公道，倾力为国内外科研院校实验室提供质产品。若有需要，我司将竭诚为您服务！
FOR RESEARCH USE ONLY
Drug Names
Generic Name：
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit 48determinations 96 determinations Storage
User manual 1 1
Closure plate membrane 2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard：360ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
wash solution （20ml×20 fold）
×1bottle （20ml×30 fold）
×1bottle 2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separately (blank comparison wells .don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
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规 格：96T/48T
检测标本：血清，血浆，尿液，胸腹水，脑脊液，细胞培养上清，组织匀浆等
检测的原理：试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）
产品的用途：仅供科研课题使用
试剂盒保存：2-8℃。
有效期：6个月
运输方法：泡沫箱配生物冰袋运输
人抗红细胞抗体（RBC）ELISA试剂盒价格及详细资料：电议,或咨询在线客服QQ741653262,或者以邮件形式发到我司邮箱qysw@qiyibio.com .齐一生物科技（上海）有限公司提供ELISA试剂盒受到了广大科研单位一致肯定和认同。大品牌保证，价格公道，倾力为国内外科研院校实验室提供质产品。若有需要，我司将竭诚为您服务！
FOR RESEARCH USE ONLY
Drug Names
Generic Name：
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit 48determinations 96 determinations Storage
User manual 1 1
Closure plate membrane 2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard：360ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
wash solution （20ml×20 fold）
×1bottle （20ml×30 fold）
×1bottle 2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separately. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separately (blank comparison wells .don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
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Cell Meter™ Caspase 3 Activity Apoptosis Assay Kit * Blue Fluorescence* 1 kit
Cell Meter™ Caspase 3 Activity Apoptosis Assay Kit * Green Fluorescence* 1 kit
Cell Meter™ Caspase 3 Activity Apoptosis Assay Kit * Red Fluorescence* 1 kit
Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit 1 kit
Cell Meter™ JC-10 Mitochondrial Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays* 1 kit
Cell Meter™ NIR Mitochondria Membrane Potential Assay Kit *Optimized for Flow Cytometry* 1 kit
Cell Meter™ NIR Mitochondria Membrane Potential Assay Kit *Optimized for Microplate Reader* 1 kit
Cell Meter™ Intracellular GSH Assay Kit *Optimized for Flow Cytometry* 1 kit
Cell Meter™ Nuclear Apoptosis Assay Kit 1 kit
Cell Meter™ Generic Fluorometric Caspase Activity Assay Kit *Optimized Green Fluorescence for Flow Cytometry* 1 kit
Cell Meter™ Generic Fluorometric Caspase Activity Assay Kit *Optimized Red Fluorescence for Flow Cytometry* 1 kit
Cell Meter™ Fluorometric Caspase 3/7 Activity Assay Kit *Green Fluorescence Optimized for Flow Cytometry* 1 kit
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Optimized Green Fluorescence for Flow Cytometry* 1 kit
Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Optimized Red Fluorescence for Flow Cytometry* 1 kit
Cell Meter™ Fluorimetric Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry* 1 kit
Cell Meter™ Fluorimetric Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry* 1 kit
Amplite™ Universal Fluorimetric Kinase Assay Kit *Red Fluorescence* 1 kit
Screen Quest™ 10X cell staining buffer with Phenol Red Plus™ 10 Plates
Screen Quest™ 10X calcium assay buffer with Phenol Red Plus™ 10 Plates
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit 1 Plate
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit 10 Plates
Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit 100 Plates
Screen Quest™ Luminometric Calcium Assay Kit 10 Plates
Screen Quest™ Luminometric Calcium Assay Kit *10X10 Plates* 100 Plates
Screen Quest™ Fluo-8 Ultra Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* 1 Plate
Screen Quest™ Fluo-8 Ultra Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* 10 Plates
Screen Quest™ Fluo-8 Ultra Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines* 100 Plates
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit 1 Plate
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit 10 Plates
Screen Quest™ Fluo-8 No Wash Calcium Assay Kit 100 Plates
Screen Quest™ Fluo-8 Ultra No Wash Calcium Assay Kit 1 Plate
Screen Quest™ Fluo-8 Ultra No Wash Calcium Assay Kit 10 Plates
Screen Quest™ Fluo-8 Ultra No Wash Calcium Assay Kit 100 Plates
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *1 plate* 1 kit
Screen Quest™ Rhod-4 No Wash Calcium Assay Kit *Medium Removal* *10 plates* 1 kit
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人抗红细胞抗体（RBC）ELISA试剂盒
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