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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
信裕生物
- 检测范围:
见说明书
- 检测方法:
Western blot/Elisa/IHC
- 应用:
科学研究
- 适应物种:
Human/Mouse/Rat
- 标记物:
见说明书
- 样本:
血清液/组织/尿
- 规格:
4 x 100 uL
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文献和实验1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
here > for recipe -MTT Lysing Solution click here > for recipe -Concanavalin A, optional (ConA, Sigma cat.no. C5275) Instruments: -Pipettes and pipettors, Multichannel pipettor -Centrifuge -37
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
: 1. Mix 20ul lO x (12.5x) LUC buffer 10ul 100mM ATP 1ul 100mg/ml BSA 169ul H20 total is 200ul/special tube for luminometer 2. add 50ul extract, mix 3. Inject 100ul diluted luciferin solution and take measurement after 5-15
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