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文献和实验Long-Range PCR with a DNA Polymerase Fusion
Proofreading DNA polymerase fusions offer several advantages for long-range PCR, including faster run times and higher fidelity compared with Taq -based enzymes. However, their use so far has been limited to amplification of small
Long-Range Polymerase Chain Reaction
fidelity of the Thermus aquaticus (Taq) DNA polymerase (3 ), the most commonly used thermostable polymerase. It is believed that inadvertent nucleotide misincorporations during the PCR extension steps cause chain terminations (3 ). The Taq polymerase
High Specificity PCR Amplification Using AccuPrime Taq DNA Polymerase
Taq DNA Polymerase 0.25 µl 0.5 µl 1 µl Autoclaved distilled water To 10 µl To 25 µl
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