Human Retinal dehydrogenase 1(

Human Retinal dehydrogenase 1(ALDH1A1) ELISA kit

标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected
800 2.127 2.139 2.133 2.035
400 1.705 1.690 1.698 1.600
200 1.205 1.232 1.219 1.121
100 0.743 0.765 0.754 0.656
50 0.485 0.468 0.477 0.379
25 0.310 0.331 0.321 0.223
12.5 0.198 0.186 0.192 0.094
0 0.099 0.096 0.098

Human Retinal dehydrogenase 1(ALDH1A1) ELISA kit

精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Human Retinal dehydrogenase 1(ALDH1A1) ELISA kit

线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ALDH1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 85
Range % 81-89
1:2 Average % 95
Range % 89-99
1:4 Average % 98
Range % 94-102
1:8 Average % 93
Range % 90-96

Human Retinal dehydrogenase 1(ALDH1A1) ELISA kit

回收率:
The recovery of human ALDH1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 85-91
EDTA plasma (n=4) 97 90-100