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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.143 2.253 2.198 2.027
20 1.544 1.534 1.539 1.368
10 1.077 1.164 1.121 0.950
5 0.774 0.792 0.783 0.612
2.5 0.513 0.523 0.518 0.347
1.25 0.368 0.398 0.383 0.212
0.625 0.273 0.281 0.277 0.106
0 0.170 0.172 0.171
Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human AKR1B10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 90
Range % 85-99
1:2 Average % 97
Range % 91-100
1:4 Average % 89
Range % 85-96
1:8 Average % 87
Range % 83-92
Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit
回收率:The recovery of human AKR1B10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 89-97
EDTA plasma (n=4) 85 81-92
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
of Tom7 (S. cerevisiae) (TOM7), mRNA 2364 NM_006039 Homo sapiens mannose receptor, C type 2 (MRC2), mRNA 564 NM_006066 Homo sapiens aldo-keto reductase family 1, member A1 (aldehyde reductase) (AKR1A1), transcript variant 1, mRNA 879 NM_013318
【资源】[共享]Adhesion Protein Protocols——（很有用的）
for the Discovery of New Adhesion Molecule Family Member... 1— Introduction 2— Materials 3— Method 4— Notes Acknowledgments References 7— Construction and Purification of Adhesion Molecule Immunoglobulin Chimeric Proteins 1— Introduction 2—
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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