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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人金葡菌肠毒素(SE)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human staphylococcus aureus enterotoxins,SE ELISA Kit
产品货号:YM-KJ1533
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人金葡菌肠毒素(SE)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人金葡菌肠毒素(SE)ELISA试剂盒 试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验大鼠sE-选择素(sE-Selectin)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 sE- 选择素 (sE-Selectin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 sE-Selectin 单抗包被于酶标板上,标准品和样品中的 sE-Selectin 与单抗结合,加入生物
超抗原对T 细胞的激活 超抗原是多种病原微生物如细菌、支原体和病毒的产物。大部分外源性超抗原来自细菌,常见的为金葡菌肠毒素(staphylococcal enterotoxln,SE)和毒性休克综合征毒素(toxic shock syndrome toxin-1,TSST―1)。病毒性超抗原为内源性,小鼠中较为常见,如小鼠乳腺肿瘤病毒(MMTV)。人体中T细胞对狂犬病毒和EB病毒的反应认为也由内源性超抗原所引起,但相应编码基因尚未被确认。 超抗原作用的特点
2.毒性疾病由金葡菌产生的有关外毒素引起 (1)食物中毒 进食含肠毒素食物后1~6小时即可出现症状,如恶心、呕吐、腹痛、腹泻,大多数病人于数小时至1日内恢复。 (2)烫伤样皮肤综合征多见于新生儿、幼儿和免疫功能低下的成人,开始有红斑,1~2天有皮起皱,继而形成水疱,至表皮脱落。由表皮溶解毒素引起。 (3)毒性休克综合征 由TSST1引起,主要表现为高热、低血压、红斑皮疹伴脱屑和休克等,半数以上病人有呕、腹泻、肌痛、结膜及粘膜充血,肝肾功能损害
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