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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人CCAAT增强子结合蛋白ε(C/EBPε)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human CCAAT/enhancer binding protein epsilon,C/EBPε ELISA Kit
产品货号:YM-KJ1311
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人CCAAT增强子结合蛋白ε(C/EBPε)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人CCAAT增强子结合蛋白ε(C/EBPε)ELISA试剂盒试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验α 672 76 PS、Ca 2+ 、DAG、FFA、LysoPC 广泛 βI 671 76 同 上 某些组织 βⅡ 673 76 同 上 多种组织 γ 697 78 同 上 脑 B组:新型PKC δ 673 77 PS、DAG 广 泛 ε 737 83 PS、DAG、FFA 脑 等 η(L) 683 77 ? 肺、皮肤、心脏 θ 707 81 ? 骨骼肌 C组:非典型PKC ζ 592 67 PS、FFA 广 泛 λ 586
的靶蛋白-DNA复合物 7)解交联,纯化富集的DNA-片断 8)PCR或基因芯片分析。 实验案例 证明C/EBP与Tmub1启动子或增强子序列的结合实验背景:在IL-6诱导条件下,利用CHiP技术提取C/EBP-DNA复合物,以Tmub1基因的碱基序列设计引物,以提取的DNA为底物,进行扩增,从而证明C/EBP结合的DNA含有Tmub1基因,进一步确认C/EBP与Tmub1启动子或增强子序列的相互作用。实验分组:分2个组进行实验普通PCR检测和WB
饥饿的 HeLa 和 NIH 3T3 癌细胞系获得的裂解物中的磷酸化蛋白特异性。图示:从复杂生物样品中富集高纯度磷蛋白。用 theThermo Scientific Pierce 磷酸化蛋白富集试剂盒进行 Weste blot 分析,根据试剂盒说明书制备细胞裂解液,富集磷酸化蛋白。利用识别生长因子信号传导相关关键调控蛋白的磷酸化特异性抗体实现蛋白检测。细胞色素 C (pI 9.6) 和 p15Ink4b (pI 5.5) 作为非磷酸化蛋白非特异性结合的阴性对照。FT = 流穿馏分,W = 合并洗脱馏分,E
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