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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人血纤蛋白(Fibrin)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human Fibrin ELISA Kit
产品货号:YM-KJ1105
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人血纤蛋白(Fibrin)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人血纤蛋白(Fibrin)ELISA试剂盒 试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验作用 (1)激肽系统:组织损伤首先激活血浆酶原Hageman因子(又称凝血因子Ⅻ),引起凝血酶级联反应。反应过程中首先是激肽释放酶(kallikrein)活化,然后产生炎症介质舒缓激肽(bradykinin)。这是激肽系统中一类血管活性碱性肽,可增加血管通透性,引起疼痛和平滑肌收缩。舒缓激肽(kinin)还可裂解C5,产生的C5a使肥大细胞释放炎症介质。 (2)凝血系统:始于血管损伤大量产生的凝血酶。凝血酶作用于血浆和组织液中的血纤蛋白原,产生血纤蛋白(fibrin)和血纤肽
|[噬菌体]尾丝抗原 fiber diffraction|纤维衍射 fiberglass|纤维玻璃 fibril|原纤维,元纤 fibrillar center|(核仁)纤维中心 fibrillarin|(核仁)纤维蛋白 fibrillin|原纤蛋白[成纤维细胞的一种胞外微原纤维蛋白] fibrillogenesis|原纤维生成 fibrin|血纤蛋白
异常而导致D-二聚体升高。 生理学背景 纤维蛋白溶解系统(fibrinolysis system)是人体最重要的抗凝系统,由4种主要部分组成:纤溶酶原(plasmingen)、纤溶酶原激活剂(plasmingen activator, 如t-PA, u-PA)、纤溶酶(plasmin)、纤溶酶抑制物(plasmin activator inhibitor, PAI-1, antiplasmin)。当纤维蛋白凝结块(fibrin clot)形成时,在tPA的存在下, 纤溶酶原激活转化为纤溶
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