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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
人黑色素瘤标记物(MART/Melan-A)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Human Melanoma Marker A,MART/Melan-A ELISA Kit
产品货号:YM-KJ0369
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,人黑色素瘤标记物(MART/Melan-A)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人黑色素瘤标记物(MART/Melan-A)ELISA试剂盒试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验。非均相 EIA 需先进行游离的和结合的标记物的分离。如前所述,固相载体可用作一种分离手段。这种固相酶免疫测定方法在 1971 年最初建立时称为酶联免疫吸附剂测定(enzymelinked immunosorbent assay),简称 ELISA,在国内有译作酶联免疫吸附试验的,虽然含义不完全确切,但已习用。
一、ELISA试剂盒的组成 完整的ELISA试剂盒包含以下各组分: (1)已包被抗原或抗体的固相载体(免疫吸附剂); (2)酶标记的抗原或抗体(结合物); (3)酶的底物; (4) 阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中); (5)结合物及标本的稀释液; (6)洗涤液,在板式ELISA中,常用的稀释液为含0.05%吐温20磷酸缓冲盐水; (7)酶反应终止
亲和素和从卵白中提取的亲和素一样,也由4条相同的肽链组成。因链霉亲和素在检测应用中发生的非特异性结合远较亲和素低,因此日渐受重视,已有取代亲和素之势。 各种抗体或酶的标记物在国内已有商品供应,使用方便。BAS ELISA 具有的特点: ①对于所有的抗原抗体系统,包括免疫细胞化学、ELISA、免疫印迹法等都适用,应用范围极广; ②BAS 在温和条件下可与各类生物大分子如蛋白质、脂多糖等结合,并且这种结合对原生物大分子的生物活性无影响; ③每个亲和素分子可与 4 个生物素分子结合,所以可以偶合更多连接
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