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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
远慕生物
- 检测范围：
科研实验
- 检测方法：
双抗体夹心酶联免疫法(ELISA)
- 样本：
血液、尿，粪便，脑脊液，胸腹水，前列腺液，精液，阴道分泌物等
- 规格：
96T/48T
人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒说明书
上海远慕生物做实验试剂，专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂！欢迎来电咨询订购：Elisa试剂盒现货低价！特价促销！
Human E-Cadherin,E-Cad ELISA Kit
产品货号：YM-KJ0021
规格：96T/48T
欢迎来电咨询订购：Elisa试剂盒，人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围：
0.2IU/L - 6IU/L
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验流式细胞仪检测益肺清化颗粒对荷瘤小鼠瘤组织E-cad CD44v6表达水平的影响
：BA0454； 上皮型钙黏附分子（E-cadherin，简作E-cad）boster公司，编号：BA0475；抗免IgG-FITC荧光标记二抗，美国Sigma公司，编号：F9887；P.B.S 0.01M pH7.2～7.4磷酸盐缓冲液，美国Gibo公司；PFA：paraformaldehyde 多聚甲醛，美国Sigma公司。1.3 药物 环磷酰胺注射液（CTX）：上海华联制药，批号：20030815。1.4实验方法 药物用法 肺瘤平膏：根据人与小鼠体表面积比系数折算，相当于人等效剂量的5倍
-钙粘着蛋白(P-Cadherin) ELISA 48T/96T 2000/3000 进口分装 F00330 环磷酸腺苷 (cAMP) ELISA 48T/96T 1500/2500 进口分装 F00340 心营养素 -1(Cardiotrophin-1) ELISA 48T/96T 2000/3000 进口分装 F00350 Caspase-1 ELISA 48T/96T 2000/3000 进口分装 F00360 Caspase-2 ELISA 48T/96T 2000
ELISA 试剂盒 货号 品 名 规格 价格 备注 F0001 人血管紧张素转化酶(Human ACE) 48T/96T 2000/3000 进口分装 F0002 激活素A(Activin A) 48T/96T 2000/3000 进口分装 F0003 ★ 人脂联素(human Adiponectin) 48T/96T 1500/2500 进口分装 F0004 白细胞活化黏附因子(ALCAM) 48T/96
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