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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
鸡单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
Chicken Herpes simplexvirus Ⅱ antibody,HSVⅡ-Ab ELISA Kit
产品货号:YM-KJ2669
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,鸡单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
鸡单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)ELISA试剂盒试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验单纯疱疹病毒(herpes simplex virus,HSV)有二个血清型,HSV-I和HSV-Ⅱ型。引起的生殖道感染的以Ⅱ型为主,约占85%.表南为生殖器官疱疹,溃疡并通过胎盘引起胎儿感染,发生死胎、流主和畸形。实验诊断多取病损处分泌物涂片进生细胞学检测、病毒培养或荧光抗体检测。在孕期感染的监测中,可采取宫颈部位分泌物做包涵体检查。单纯疱疹病毒以侵犯军团颈鳞状上皮多见。感染早期细胞轻度或中度增大,核呈嗜碱性不透明的匀质状毛玻璃样外观,偶伴有核空泡化。由于核的增殖与胞质肿大而形成多核
TORCH是指一组病原微生物的英文名称缩写。TO即(刚地弓形虫或弓形虫),R即(风疹病毒),C即(巨细胞病毒),H即(单纯疱疹病毒Ⅰ型和Ⅱ型)。 这组病原体常可通过胎盘传给胎儿,引起围产期感染,导致流产、死胎、早产、先天畸形和智力障碍等各种异常结果,因此受到广泛关注。TORCH感染的抗体检查在许多地区已作为孕期检查的常规项目。 一、感染TORCH对胎儿有哪些影响: 1、刚地弓形虫 弓形虫感染是一种人畜共患疾病,广泛分布于世界各地。由猫与其他宠物传染人的可能性较大
佚名 此病毒为DNA病毒,其中能经起中枢神经系统感染者至少有四种:①单纯疱疹病毒(HSV),②带状疱疹病毒,③EB病毒,④巨细胞病毒,其中以单纯疱疹病毒感染最为多见。 单纯疱疹病毒脑炎是欧美常见的散发性脑炎,可见于新生儿(HSVⅡ型)或见于儿童和青年(HSVⅠ型)。发生在成人中的急性坏死性脑炎病情凶险,死亡率为30%~70%。病变多累及一侧或双侧颞叶
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