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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
远慕生物
- 检测范围:
科研实验
- 检测方法:
双抗体夹心酶联免疫法(ELISA)
- 样本:
血液、尿,粪便,脑脊液,胸腹水,前列腺液,精液,阴道分泌物等
- 规格:
96T/48T
兔子血纤蛋白原降解产物(FDP)ELISA试剂盒说明书
上海远慕生物做实验试剂,专业供应Elisa试剂盒、生物试剂、生化试剂、抗体、培养基、标准品|对照品等科研试剂!欢迎来电咨询订购:Elisa试剂盒现货低价!特价促销!
rabbit Fibrinogen Degradation Product,FDP ELISA Kit
产品货号:YM-KJ2391
规格:96T/48T
欢迎来电咨询订购:Elisa试剂盒,兔子血纤蛋白原降解产物(FDP)ELISA检测试剂盒
Kit composition:
Closure plate membrane: 2 (48) /2 (96)
Instruction: 1
Sealed bag: 1
Standard product: 0.5ml 2700ng/L * 1 0.5ml * 1 2-8
Enzyme labeled plate: 1 * 481 * 96 * 2-8
Sample dilution: 3ml * 1 ml * 1 2-8
Color reagent A solution: 3ml * 6 ml * 1 2-8
Color reagent B solution: 3ml * 6 ml * 1 2-8
Termination liquid: 3ml * 1 6ml * 1 2-8
Concentrated washing liquid: (20ml * 20) x 1 (20ml * 30) x 1 2-8
Objective: this kit is used to determine the content of serum, plasma and related liquid samples.
Operation steps:
Dilution and addition of 1 standard products:
2 samples: respectively, the blank hole (blank control hole without the sample and the enzyme label, and the remaining steps are the same), the sample hole.
3 temperature Education: 37 minutes after the closure of the sealing plate with a sealing plate.
4 with liquid: 30 (48T 20 times) the concentrated detergent liquid with distilled water 20 (48T 30 times) after dilution.
5. Washing: be careful torn off the seal plate membrane, discard liquid, drying, washing liquid to fill each hole, standing for 30 seconds after the discard, repeat 5 times, pat dry.
6 enzyme: 50 L per hole, except for the blank. 7 Wen Yu: operation with 3.
8 washing: operation with 5.
9 Color: first add color agent A50 L, and then add color B50 L, gently shake the mixture, 37 to 15 minutes.
10 termination: 50 L per hole with the end of the end of the reaction (at this time, blue).
11: Determination of the blank absorbance wavelength of 450nm zero air conditioning, in order to measure the hole (OD). Determination should be carried out within 15 minutes after the termination of the liquid.
兔子血纤蛋白原降解产物(FDP)ELISA试剂盒试剂盒性能:
1.样品线性回归与预期浓度相关系数R值为0.95以上。
2.批内与批见应分别小于9%和11%
检测范围:
0.2IU/L - 6IU/L
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文献和实验) ELISA 48T/96T 2000/3000 进口分装 F20030 兔内皮素 -1(rabbit ET-1) ELISA 48T/96T 2000/3000 进口分装 F20040 兔纤维蛋白原降解产物 (rabbit FDP
【参考值】孵育前10~12秒,孵育60分钟<30秒。 【临床意义】 当体内肝素和纤维蛋白降解产物(FDP)增多,纤维蛋白原减少时未孵育的凝血酶时间延长医学教育网搜|索整理。由于血浆中有纤维蛋白溶解活化素及血浆素原(纤溶酶原)存在,在孵育过程中血浆素原转变为血浆素,将纤维蛋白原水解为纤维蛋白原降解产物,该产物拮抗凝血酶使凝血酶凝固时间延长。当血浆中活化素增高时,纤维蛋白降解产物增加明显,孵育后凝血酶时间明显延长超出正常范围,对诊断DIC是敏感指标,假阳性少见。此外在原发
【参考值】 孵育前10~12秒,孵育60分钟<30秒。 【临床意义】 当体内肝素和纤维蛋白降解产物(FDP)增多,医学教育|网搜集整理纤维蛋白原减少时未孵育的凝血酶时间延长。由于血浆中有纤维蛋白溶解活化素及血浆素原(纤溶酶原)存在,在孵育过程中血浆素原转变为血浆素,将纤维蛋白原水解为纤维蛋白原降解产物,该产物拮抗凝血酶使凝血酶凝固时间延长。当血浆中活化素增高时,纤维蛋白降解产物增加明显,孵育后凝血酶时间明显延长超出正常范围,对诊断DIC是敏感指标,假阳性少见
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