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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Human angiotensin Ⅱ receptor 1,ANGⅡR-1 ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.354 2.254 2.304 2.153
1500 1.712 1.612 1.662 1.511
750 1.131 1.081 1.106 0.955
375 0.566 0.556 0.561 0.410
187.5 0.338 0.328 0.333 0.182
93.75 0.277 0.267 0.272 0.121
46.88 0.209 0.205 0.207 0.056
0 0.152 0.150 0.151
Human angiotensin Ⅱ receptor 1,ANGⅡR-1 ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human angiotensin Ⅱ receptor 1,ANGⅡR-1 ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human ANGⅡR-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 92
Range % 81-98
1:2 Average % 96
Range % 93-105
1:4 Average % 94
Range % 87-98
1:8 Average % 103
Range % 96-110
Human angiotensin Ⅱ receptor 1,ANGⅡR-1 ELISA Kit
回收率:The recovery of human ANGⅡR-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-96
EDTA plasma (n=4) 99 90-108
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文献和实验Characterization of Angiotensin II Receptors
and histidine‐256 (HISVI‐16) of the angiotensin type 1 receptor. Mol. Pharmacol. 47:425‐431. Smith, R.D., and Timmermans, P.B.M.W.M. 1994. Human angiotensin receptor subtypes. Curr
特异性的引物PCR扩增。基因经过30-35个循环的一轮PCR扩增。50μl PCR扩增产物中的15μl在1.2％E-Gel®琼脂糖凝胶电泳检测，在紫外光下观察。 泳道1：HPRT（次黄嘌呤磷酸核糖转移酶），1.3kb，30拷贝/细胞 泳道2：SMAP（胸腺激素受体共激活蛋白），3.1kb 泳道3：Cleavage and polyadenylation specificity factor，4.5kb 泳道4：TFRC（转铁蛋白受体），5.0kb 泳道5：IGFⅡR（类胰岛素生长因子Ⅱ受体
农（milrinone）；②血管扩张药（Vasodilators）；③b- 受体阻断药（b-blockers）；④血管紧张素转化酶抑制药（angiotensin converting enzyme inhibitor，ACEI）或AngⅡ受体阻断药（AngⅡ receptor blockers ）；⑤醛固酮拮抗药（Aldosterone antagonists）；⑥利尿药（Diuretics）等。其作用环节如图25-1所示。 正性肌力药 编辑本段 回目录 强心苷
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