Catalog # / Size: 752602 / 10 µg
752604 / 25 µg
752606 / 100 µg
Source: Mouse TNFSF18, amino acids (Thr47 - Ser173) (Accession# NM_005092), was expressed in 293E cells. The amino-terminal
possesses a linker-9His tag.
Molecular Mass: The 148 amino acid recombinant protein has a predicted molecular mass of approximately 16.7 kD. The protein migrates at approximately 20-30kD in both DTT-reducing and non-reducing conditions by SDS-PAGE.
The predicted N-terminal amino acid is His.
Purity: >95%, as determined by Coomassie stained SDS-PAGE.
Endotoxin Level: Less than 0.01 ng per µg cytokine as determined by the LAL method.
Activity: ED50 = 0.05 - 0.4 µg/mL as determined by the induction of splenocytes proliferation when anti-mouse CD3 (200 ng/well, Cat. No. 100207) and TNFSF18 are immobilized.
Formulation: 0.22 µm filtered protein solution is in PBS, pH 7.4.
Concentration: 10-100 µg sizes are bottled at 200 µg/mL.
Storage: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one
year. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C to
-70°C. Stock solutions can also be prepared at 50 - 100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS)
containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid
repeated freeze/thaw cycles.