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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Mouse adrenocorticotropic hormone (ACTH) ELISA kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1200 3.134 3.126 3.130 2.988
600 2.537 2.532 2.535 2.393
300 1.754 1.765 1.760 1.618
150 1.053 1.050 1.052 0.910
75 0.651 0.647 0.649 0.507
37.5 0.443 0.435 0.439 0.297
18.75 0.273 0.265 0.269 0.127
0 0.140 0.144 0.142
Mouse adrenocorticotropic hormone (ACTH) ELISA kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Mouse adrenocorticotropic hormone (ACTH) ELISA kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 92
Range % 80-105
1:2 Average % 96
Range % 85-104
1:4 Average % 98
Range % 92-104
1:8 Average % 96
Range % 82-110
Mouse adrenocorticotropic hormone (ACTH) ELISA kit
回收率:The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 84-102
EDTA plasma (n=4) 98 90-105
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Studying Wound Repair in the Mouse
in a reproducible, controlled environment. Here, we detail methods for full?thickness excisional and incisional wounding and for analysis of wound biopsies. Curr. Protoc. Mouse Biol . 3:171?185 © 2013 by John Wiley & Sons, Inc. Keywords: wound
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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