精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ATP1A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1 Average % 96
Range % 90-100
1:2 Average % 104
Range % 100-108
1:4 Average % 84
Range % 80-88
1:8 Average % 90
Range % 86-94
回收率:
The recovery of mouse ATP1A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 94 90-98