精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Human alpha-actinin-4(ACTN-4) ELISA Kit
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ACTN-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:100 Average % 90
Range % 84-96
1:200 Average % 90
Range % 84-95
1:400 Average % 99
Range % 95-105
1:800 Average % 94
Range % 89-99
Human alpha-actinin-4(ACTN-4) ELISA Kit
回收率:
The recovery of human ACTN-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 87-96
EDTA plasma (n=4) 99 90-104