PRINCIPLE OF THE ASSAY
This assay employs the competitive inhibition enzyme immunoassay technique.
The microtiter plate provided in this kit has been pre-coated with an antibody
specific to AChE. Standards or samples are added to the appropriate microtiter
plate wells with Biotin-conjugated AChE. A competitive inhibition reaction is
launched between AChE (Standards or samples) and Biotin-conjugated AChE
with the pre-coated antibody specific for AChE. The more amount of AChE in
samples, the less antibody bound by Biotin-conjugated AChE. After washing,
avidin conjugated Horseradish Peroxidase (HRP) is added to the wells.
Substrate solution is added to the wells and the color develops in opposite to the
amount of AChE in the sample. The color development is stopped and the
intensity of the color is measured.
Fish Acetylcholinesterase(AChE)ELISA Kit
DETECTION RANGE
2.5 ng/ml-40 ng/ml.
Fish Acetylcholinesterase(AChE)ELISA Kit
SENSITIVITY
The minimum detectable dose of fish AChE is typically less than 1.25 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as
the lowest fish AChE concentration that could be differentiated from zero. It was
determined the mean O.D value of 20 replicates of the zero standard added by
their three standard deviations.
Fish Acetylcholinesterase(AChE)ELISA Kit
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of fish AChE.
No significant cross-reactivity or interference between fish AChE and analogues
was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between fish AChE and all the analogues, therefore,
cross reaction may still exist.
3
PRECISION
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to
assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
LIMITATIONS OF THE PROCEDURE
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES.
The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the
samples and repeat the assay.
Any variation in operator, pipetting technique, washing technique,
incubation time or temperature, and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors,
binding proteins, and other factors present in biological samples. Until all
factors have been tested in the Immunoassay, the possibility of
interference cannot be excluded.