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文献和实验Application of TEV Protease in Protein Production
method for the removal of affinity tags from recombinant fusions using a highly purified proteinase with an unparalleled degree of specificity. This proteinase, from the genome of tobacco etch virus (TEV), demonstrates specific proteolytic activity
RNA-Protein Crosslink Mapping Using TEV Protease
specific TEV protease from tobacco etch virus. Covalent RNA-protein crosslinks can then be physically mapped by TEV protease digestion, fractionation of the proteolytic digestion products by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
细胞功能抑制因子,可以减弱宿主细胞免疫监视功能,促进肿瘤免疫逃逸的作用机制。该文章使用了 Vazyme Tn5 转座酶(TD501)进行 ATAC-seq 实验以及 PA-Tn5(S603)进行 CUT&Tag 实验。 参考文献: [1] Vaezeslami S , Sterling R , Reznikoff W S . Site-Directed Mutagenesis Studies of Tn5 Transposase Residues Involved in Synaptic
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