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文献和实验Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis
or, if combined with the use of a specific promoter, in a time- and cell-type-dependent manner. The most widely used approach to obtain this regulation relies on the fusion of the recombinase, mostly Cre recombinase, with the ligand-binding domain (LBD
Conditional Gene Knockout Using Cre Recombinase
The directed introduction of null mutations into defined genes has proven invaluable in elucidating gene function in a variety of experimental organisms. In the last decade or so this approach has been extended to mice (1 ) by the combined
Creation and Use of a Cre Recombinase Transgenic Database
Embryonic stem (ES) cell-mediated transgenic approaches have revolutionized mammalian genetics in the last decade. Close to 4000 genes have been targeted to date. Analysis of these genetic alterations has provided an unprecedented
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