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文献和实验Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay
The procedure described here, the PCR poly(A) test (PAT), allows the analysis of the poly(A) tail on a specific mRNA within a pool of total RNA in a rapid (1 day) and sensitive (subnanogram total RNA) fashion. The assay also provides
Signals for Retention of Cytochrome P450 in the Endoplasmic Reticulum
amino acid sequence such as a double lysme motif at the C-terminus of the cytoplasmic tail or positively charged residues at the N-terminus (reviewed in ref. 1 ). ER retention signals may mediate either the direct retention (exclusion
Visualization of Peptide Secretory Vesicles in Living Nerve Cells
of overexpression of exogenous peptides comprising of the cytoplasmic tail sequence of vesicular carboxypeptidase E (CPE), proposed to be involved in the mechanism of trafficking of peptidergic secretory vesicles, in live hippocampal neurons
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