Recombinant Human Vacuolar Protein Sorting 24
Synonyms Charged multivesicular body protein 3, Chromatin-modifying protein 3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24, CHMP3, CGI149, NEDF, VPS24, CGI-149.
Introduction Charged multivesicular body protein 3 (VPS24/CHMP3) is a member of the vacuolar sorting protein family and function as chromatin modifying proteins. VPS24 links directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. VPS24 overexpression strongly hinders HIV-1 release.
Recombinant Human Vacuolar Protein Sorting 24Description VPS24 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 242 amino acids (1-222 a.a) and having a molecular mass of 27.2kDa (Molecular weight on SDS-PAGE will appear higher).
VPS24 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered colorless solution.
Recombinant Human Vacuolar Protein Sorting 24Formulation VPS24 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability VPS24 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Purity Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS.
Recombinant Human Vacuolar Protein Sorting 24Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.