Recombinant Mouse BH3 Interacting Domain Death Agonist p15
Synonyms Truncated BH3-interacting domain death agonist, p22 BID, BID, FP497, MGC15319,
MGC42355, tBID.
Introduction Truncated BH3 interacting domain death agonist is a truncated form of the pro-apoptotic full-length BID. Truncated BH3 interacting domain death agonist is generated by Caspase-8 cleavage of BID. The truncated form of the protein translocatesfrom the cytosol to mitochondria and transduces apoptotic signals.
BID is a pro-apoptotic Bcl-2 protein having only the BH3 domain. In reaction to apoptotic signaling, BID interacts with another Bcl-2 family of cell death regulators, called Bax, they form a heterodimer resulting to the insertion of Bax into the outer mitochondrial membrane. Bax induces the opening of the mitochondrial voltage-dependent anion channel which lead to the release of cytochrome c and other pro-apoptotic factors from the mitochondria resulting in activation of caspases. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8). CASP8 cleaves BID, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. The major proteolytic product p15 BID releasea cytochrome c. Isoform 1, Isoform 2 and Isoform 4 induce ice-like proteases and apoptosis while Isoform 3 does not induce apoptosis.
Recombinant Mouse BH3 Interacting Domain Death Agonist p15Description Truncated BID Mouse Recombinant also called BH3-interacting domain death agonist p15 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 61-195 amino acids (135 a.a.) and having a molecular mass of 15.4 kDa.
Source Escherichia Coli.
Physical Appearance Sterile Filtered colorless liquid formulation.
Recombinant Mouse BH3 Interacting Domain Death Agonist p15Formulation The Mouse Truncated BID protein solution contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
Stability tBID although stable at 15°C for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Recombinant Mouse BH3 Interacting Domain Death Agonist p15Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.