Human Apolipoprotein F(APOF) E

Human Apolipoprotein F(APOF) ELISA kit

PRINCIPLE OF THE ASSAY
This assay employs the competitive inhibition enzyme immunoassay technique.
The microtiter plate provided in this kit has been pre-coated with APOF.
Standards or samples are added to the appropriate microtiter plate wells with
Horseradish Peroxidase (HRP) conjugated antibody preparation specific for
APOF. The competitive inhibition reaction is launched between with pre-coated
APOF and APOF in samples. A substrate solution is added to the wells and the
color develops in opposite to the amount of APOF in the samples. The color
development is stopped and the intensity of the color is measured.

Human Apolipoprotein F(APOF) ELISA kit

DETECTION RANGE
9.38 ng/ml-600 ng/ml.

Human Apolipoprotein F(APOF) ELISA kit

SENSITIVITY
The minimum detectable dose of human APOF is typically less than 2.34 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as
the lowest protein concentration that could be differentiated from zero. It was
determined the mean O.D value of 20 replicates of the zero standard added by
their three standard deviations.
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of
human APOF. No significant cross-reactivity or interference between human
APOF and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between human APOF and all the analogues,
therefore, cross reaction may still exist.

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Human Apolipoprotein F(APOF) ELISA kit

PRECISION
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to
assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
LIMITATIONS OF THE PROCEDURE
 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES.
 The kit should not be used beyond the expiration date on the kit label.
 Do not mix or substitute reagents with those from other lots or sources.
 If samples generate values higher than the highest standard, dilute the
samples with Sample Diluent and repeat the assay.
 Any variation in Sample Diluent, operator, pipetting technique, washing
technique, incubation time or temperature, and kit age can cause variation
in binding.
 This assay is designed to eliminate interference by soluble receptors,
binding proteins, and other factors present in biological samples. Until all
factors have been tested in the Immunoassay, the possibility of
interference cannot be excluded.