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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Rat Apolipoprotein C-III(APOC3) ELISA kit
PRINCIPLE OF THE ASSAYThis assay employs the competitive inhibition enzyme immunoassay technique.
The microtiter plate provided in this kit has been pre-coated with APOC3.
Standards or samples are added to the appropriate microtiter plate wells with
Horseradish Peroxidase (HRP) conjugated antibody preparation specific for
APOC3. The competitive inhibition reaction is launched between with pre-coated
APOC3 and APOC3 in samples. A substrate solution is added to the wells and
the color develops in opposite to the amount of APOC3 in the samples. The color
development is stopped and the intensity of the color is measured.
Rat Apolipoprotein C-III(APOC3) ELISA kit
DETECTION RANGE31.25 ng/ml-2000 ng/ml.
Rat Apolipoprotein C-III(APOC3) ELISA kit
SENSITIVITYThe minimum detectable dose of rat APOC3 is typically less than 7.8 ng/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as
the lowest protein concentration that could be differentiated from zero. It was
determined the mean O.D value of 20 replicates of the zero standard added by
their three standard deviations.
Rat Apolipoprotein C-III(APOC3) ELISA kit
SPECIFICITYThis assay has high sensitivity and excellent specificity for detection of
rat APOC3. No significant cross-reactivity or interference between rat APOC3
and analogues was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between rat APOC3 and all the analogues,
therefore, cross reaction may still exist.
3
PRECISION
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to
assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
LIMITATIONS OF THE PROCEDURE
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES.
The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the
samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing
technique, incubation time or temperature, and kit age can cause variation
in binding.
This assay is designed to eliminate interference by soluble receptors,
binding proteins, and other factors present in biological samples. Until all
factors have been tested in the Immunoassay, the possibility of
interference cannot be excluded.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
azide 3 × 106 beads/mL in deionized water containing 2 mM sodium azide and 0.05% Tween® 20 2–6°C DO NOT FREEZE When stored as directed, this kit is stable
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
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