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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
human Fetuin A ELISA Kit
标准曲线:These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
1000 2.754 2.797 2.776 2.564
500 2.312 2.354 2.333 2.121
250 1.563 1.554 1.559 1.347
125 0.735 0.753 0.744 0.532
62.5 0.538 0.526 0.532 0.320
31.2 0.322 0.335 0.329 0.117
15.6 0.268 0.284 0.276 0.064
0 0.211 0.213 0.212
human Fetuin A ELISA Kit
精密度:Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
human Fetuin A ELISA Kit
线性度:To assess the linearity of the assay, samples were spiked with high concentrations of human fetuin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:1000 Average % 95
Range % 90-100
1:2000 Average % 94
Range % 91-99
1:4000 Average % 92
Range % 88-98
1:8000 Average % 93
Range % 84-102
human Fetuin A ELISA Kit
回收率:The recovery of human fetuin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 92-106
EDTA plasma (n=4) 98 91-105
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
Solid-Phase Assays of Receptor-Binding Specificity
between animal species leading to variations in the receptor-binding specificity of viruses circulating in these species. Analysis of the receptor specificity of different animal and human influenza viruses can give insight into factors and mechanisms
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