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- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
康朗生物
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
HRP标记物
- 样本：
血清/组织/尿液
Rat ATP-citrate synthase(ACLY) ELISA kit
PRINCIPLE OF THE ASSAYThis assay employs the quantitative sandwich enzyme immunoassay technique.
Antibody specific for ACLY has been pre-coated onto a microplate. Standards
and samples are pipetted into the wells and any ACLY present is bound by the
immobilized antibody. After removing any unbound substances, a
biotin-conjugated antibody specific for ACLY is added to the wells. After washing,
avidin conjugated Horseradish Peroxidase (HRP) is added to the wells.
Following a wash to remove any unbound avidin-enzyme reagent, a substrate
solution is added to the wells and color develops in proportion to the amount of
ACLY bound in the initial step. The color development is stopped and the
intensity of the color is measured.
Rat ATP-citrate synthase(ACLY) ELISA kit
DETECTION RANGE37.5 pg/ml-2400 pg/ml.
Rat ATP-citrate synthase(ACLY) ELISA kit
SENSITIVITYThe minimum detectable dose of rat ACLY is typically less than 9.37 pg/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as
the lowest protein concentration that could be differentiated from zero. It was
determined the mean O.D value of 20 replicates of the zero standard added by
their three standard deviations.
Rat ATP-citrate synthase(ACLY) ELISA kit
SPECIFICITYThis assay has high sensitivity and excellent specificity for detection of rat ACLY.
No significant cross-reactivity or interference between rat ACLY and analogues
was observed.
Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between rat ACLY and all the analogues, therefore,
cross reaction may still exist.
3
PRECISION
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to
assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
LIMITATIONS OF THE PROCEDURE
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC
PROCEDURES.
The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the
samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing
technique, incubation time or temperature, and kit age can cause variation
in binding.
This assay is designed to eliminate interference by soluble receptors,
binding proteins, and other factors present in biological samples. Until all
factors have been tested in the Immunoassay, the possibility of
interference cannot be excluded.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
of your primary antibody. 1) Heat Induced Epitope Retrieval Protocol 2) Based on the reagent that you are using, dilute the reagent to 1X as follows: To 25 ml 20×Citrate Buffer, pH 6.0 solution, add 475 ml distilled water
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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