Recombinant Human Frizzled-1
Synonyms Frizzled-1, Fz-1, hFz1, FzE1, FZD1, FLJ95923, DKFZp564G072.
Introduction “Frizzled” gene family members encode 7-transmembrane domain proteins which are receptors for Wnt signaling proteins. The FZD1 protein includes a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in the adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in the fetal lung and kidney.
Recombinant Human Frizzled-1Description Frizzled-1 Human Recombinant produced in HEK293 cells, corresponding to the extracellular region of mature Human Frizzled-1 (amino acids 112-228) and fused to the Fc region of Human IgG1, having an MW of 46kDa.
Frizzled-1 is purified by proprietary chromatographic techniques.
Source HEK293 (Human Embryonic Kidney cell line).
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized without any additives.
Recombinant Human Frizzled-1Solubility It is recommended to reconstitute the lyophilized FZD1 in sterile 1xPBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized FZD1 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution FZD1 should be stored at 4℃ between 2-7 days and for future use below -18℃.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence DHGYCQPISI PLCTDIAYNQ TIMPNLLGHT NQEDAGLEVH QFYPLVKVQC SAELKFFLCS MYAPVCTVLE QALPPCRSLC ERARQGCEAL MNKFGFQWPD TLKCEKFPVH GAGELCVENL YFQGGSGTKL DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHGD PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGK.
Recombinant Human Frizzled-1Biological Activity The KD (equilibrium binding constant) of FZD1 Human was measured at less than 10nM with human Wnt3a as the ligand. The measurements were performed by the ForteBio Octet optical biosensor instrument.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.