Bovine Angiopoietin-1(ANGPT1) ELISA kit PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANGPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Bovine Angiopoietin-1(ANGPT1) ELISA kit DETECTION RANGE 12.5 ng/ml-800 ng/ml. SENSITIVITY The minimum detectable dose of bovine ANGPT1 is typically less than 1.384 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Bovine Angiopoietin-1(ANGPT1) ELISA kit SPECIFICITY This assay has high sensitivity and excellent specificity for detection of bovine ANGPT1. No significant cross-reactivity or interference between bovine ANGPT1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between bovine ANGPT1 and all the analogues, therefore, cross reaction may still exist. 3 PRECISION Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. LIMITATIONS OF THE PROCEDURE FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC Bovine Angiopoietin-1(ANGPT1) ELISA kit PROCEDURES. The kit should not be used beyond the expiration date on the kit label. Do not mix or substitute reagents with those from other lots or sources. If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay. Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding. This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.