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Recombinant Human B-Cell Leuke

mia/Lymphoma 2 -NWGR
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  • ¥1680 - 3988
  • 美国ATCC
  • 美国/中国
  • 2025年12月23日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 供应商

      钰博生物

    • 细胞类型

      复苏

    • 组织来源

      见说明书

    • 物种来源

      人、大鼠、小鼠、、、、

    • 免疫类型

      请咨询销售人员

    • 细胞形态

      上皮样;多角形

    • 是否是肿瘤细胞

      见说明书

    • 器官来源

      见说明书

    • 运输方式

      干冰运输or活细胞运输

    • 年限

      见说明书

    • 生长状态

      贴壁生长

    Recombinant Human B-Cell Leukemia/Lymphoma 2 -NWGR
    Synonyms Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
    Introduction BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.
    Description Bcl-2 Des NWGR domain (143-146 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 214 amino acids 1-142 and 147-218.
    The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.
    Recombinant Human B-Cell Leukemia/Lymphoma 2 -NWGRSource Escherichia Coli.
    Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
    Formulation The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.
    Solubility Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4°C.
    Dilute 10 fold into selected buffer system.
    BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
    Recombinant Human B-Cell Leukemia/Lymphoma 2 -NWGRStability Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below
    -18°C.
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please prevent freeze-thaw cycles.
    Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.
    Applications Input marker or positive control (Western Blotting).
    Function study.
    Recombinant Human B-Cell Leukemia/Lymphoma 2 -NWGRUsage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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