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- PEPROTECH
- 中国/美国
- xy-562Ra01
- 2026年01月22日
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Never In Mitosis Gene A Related Kinase 2 (NEK2)
- 库存：
1000
- 供应商：
上海信裕
永离有丝分裂基因A相关激酶2(NEK2)重组蛋白
Recombinant Never In Mitosis Gene A Related Kinase 2 (NEK2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy562Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 34.1kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
永离有丝分裂基因A相关激酶2(NEK2)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-VLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTPYYMSPEQISR LSYNEKSDIW SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLQDSSPVLSELKLKE RQLQDRERAL RAREDSLEQK ERELCIRERL AEDKLARAES LLKNYSLLKEHRLLCLAGGS ELDLPSSALK KKVHFHGESK ENTARSENSE
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-VLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTPYYMSPEQISR LSYNEKSDIW SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLQDSSPVLSELKLKE RQLQDRERAL RAREDSLEQK ERELCIRERL AEDKLARAES LLKNYSLLKEHRLLCLAGGS ELDLPSSALK KKVHFHGESK ENTARSENSE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
永离有丝分裂基因A相关激酶2(NEK2)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
永离有丝分裂基因A相关激酶2(NEK2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
永离有丝分裂基因A相关激酶2(NEK2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
相关专题 无血清培养基产品选择指南 Serum-free media(SFM) 技术介绍 1、含血清成分培养体系的劣势 培养动物细胞，无论是为了增殖病毒，还是为了获得相关的生物制品，都力求使细胞大规模、高密度生长，并在高密度下维持高的细胞活性，简化下游纯化工艺，提高生物制品的安全性和产率，降低产品的成本，提高市场竞争力。但是常规细胞培养 基的组分——血清的存在严重制约了动物细胞的规模培养，使用户不得不每次
才能实现，发酵工程和酶工程有的也与细胞培养密切相关。总之，细胞培养在整个生物技术产业的发展中起到了很关键的核心作用。第一节 体外培养的概念一、基本概念 体外培养（in vitro culture），就是将活体结构成分或活的个体从体内或其寄生体内取出，放在类似于体内生存环境的体外环境中，让其生长和发育的方法。组织培养：是指从生物体内取出活的组织（多指组织块）在体外进行培养的方法。细胞培养：是指将活细胞（尤其是分散的细胞）在体外进行培养的方法。器官培养：是指从生物体内取出的器官（一般是胚胎器官）、器官
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永离有丝分裂基因A相关激酶2(NEK2)重组蛋白
¥1 - 3980
