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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
4°C, sealed storage, away from moisture
- 英文名:
Direct Blue 53; T-1824; C.I. 23860
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
314-13-6
- 规格:
10 mM * 1 mL/100 mg/500 mg/1 g/5 g
| 规格: | 10 mM * 1 mL | 产品价格: | ¥550.0 |
|---|---|---|---|
| 规格: | 100 mg | 产品价格: | ¥500.0 |
| 规格: | 500 mg | 产品价格: | ¥880.0 |
| 规格: | 1 g | 产品价格: | ¥1056.0 |
| 规格: | 5 g | 产品价格: | ¥1760.0 |
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Evans Blue
CAS No. : 314-13-6
MCE 国际站:Evans Blue
产品活性:Evans Blue (Direct Blue 53) 是一种通过膜结合兴奋性氨基酸转运蛋白 (EAAT) 摄取 L-谷氨酸的有效抑制剂。Evans Blue 是一种 L-谷氨酸 (L-glutamate) 和红藻氨酸受体 (kainate receptor) 介导的电流抑制剂。Evans Blue 对血清白蛋白具有很强的亲和力,使其成为一种高分子量蛋白质示踪剂。Evans Blue 还用于研究 BBB(血脑屏障)渗透性。
研究领域:Membrane Transporter/Ion Channel | Neuronal Signaling
In Vitro: Evans blue, a bis-azo dye, is a non-permeating dye. In presence of plasma membrane damage, Evans blue enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability.
In Vivo: The amount of Evans Blue (2% solution; 4 mL/kg) stain accumulated in the brains of mice after ICH produced by collagenase injection was independent of the stain administration route.
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文献和实验, we aimed to highlight the basic knowledge on the use of three most commonly performed tracers, namely Evans blue dye, sodium fluorescein, and horseradish peroxidase. The experimental methodologies that we use in our laboratory for the detection
心包膜,将心脏推出体外,暴露左冠状动脉前降支,用6-0无菌丝线快速结扎,打活结,看到心脏底部变白,用荷包缝法迅速关闭胸腔,挤压防止气胸,造成左室前壁心肌缺血。分别记录术前、缺血时心电图变化。 到达缺血时间以后,解开活结,再次关闭胸腔,缝合肌肉皮肤,进行心肺复苏。分别记录术前、缺血时和再灌注后的心电图变化。造模成功后进行药物治疗,术后观察动物活动、采食情况。处死前对实验动物采血。术后进行心肌组织取样、TTC-Evans blue染色,进行心梗面积计算。 4、实验操作及检测
DNA laddering assay for treated cells
describe in their paper (Gong et al., 1994, Anal. Biochem., 218: 314-19), this procedure is simple and uses nontoxic reagents (no phenol, chloroform used). The cells are prefixed in 70% ethanol and, if wished, can be stored in this way for several weeks before analysis
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