Recombinant Human Carbonic Anhydrase III
Synonyms Car3, CAIII, Carbonic anhydrase 3, EC 4.2.1.1, Carbonic anhydrase III, Carbonate dehydratase III, CA-III.
Introduction Carbonic anhydrase III is part of a multigene family that encodes carbonic anhydrase isozymes which are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in various cell types. Carbonic anhydrase III expression is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. CA3 catalyses swift conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O = HCO3 + H+). CA3 participates in a variety of biological processes, including respiration, calcifica-tion, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. CA3 includes a zinc ion in its active site and maintains acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues.
Recombinant Human Carbonic Anhydrase IIIDescription CA3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 260 amino acids (1-260 a.a.) and having a molecular mass of 29.5 kDa. The CA3 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered clear colorless solution.
Formulation The CA3 solution contains 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol.
Recombinant Human Carbonic Anhydrase IIIStability CA3 Recombinant Human although stable at 4°C for 30 days, should be stored desiccated below -20°C for periods greater than 30 days. Please avoid freeze-thaw cycles.
Amino acid sequence MAKEWGYASH NGPDHWHELF PNAKGENQSP IELHTKDIRH DPSLQPWSVS YDGGSAKTIL NNGKTCRVVF DDTYDRSMLR GGPLPGPYRL RQFHLHWGSS DDHGSEHTVD GVKYAAELHL VHWNPKYNTF KEALKQRDGI AVIGIFLKIG HENGEFQIFL DALDKIKTKG KEAPFTKFDP SCLFPACRDY WTYQGSFTTP PCEECIVWLL LKEPMTVSSD QMAKLRSLLS SAENEPPVPL VSNWRPPQPI NNRVVRASFK.
Recombinant Human Carbonic Anhydrase IIIPurity Greater than 90% as determined by SDS-PAGE.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.