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125g
SeaKem® Gold Agarose 琼脂糖粉 125g
| 产品应用: | DNA/RNA电泳制备、分离,DNA/RNA杂交,PCR反应分析 | ||
| 中国俗称: | Agarose 琼脂糖粉 | 中国简称: | 琼脂糖 |
| 注册品牌: | LONZA | 生产厂家: | 美国Lonza公司 |
| 订购货号: | 50150 | 保存温度: | 室温 |
| 规格型号: | 125g | ||
| 产品种类: | 琼脂糖 | ||
| 到货时间: | 上海仓库有现货,1~3天到 | ||
产品英文名称:SeaKem® Gold Agarose
产品中文名称:SeaKem® Gold琼脂糖粉
SeaKem® Gold Agarose琼脂糖 产品介绍:
SeaKem® Gold Agarose是一种高凝胶强度,低EEO(电渗),标准凝胶温度的琼脂糖。SeaKem® Gold Agarose属于遗传技术级别, 最适于在脉冲场凝胶电泳实验(PFGE)中快速分离巨大碱基对DNA。低电渗性能使得其在凝胶电泳实验中DNA电泳迁移率明显大于使用传统琼脂糖,调节缓 冲液和琼脂糖浓度可以使跑胶时间减少一半(具体方法参照说明书)。高凝胶强度使其在低浓度时很容易操作,能够使用传统电泳分离大DNA片段,减少分离 DNA片段时间(PFGE)。
SeaKem® Gold Agarose 产品参数:
› Gelling temperature (1.5%) ――36°C ±1.5°C
› Melting temperature (1.5%)―― >90°C
› Gel strength (1%)―― >1,800 g/cm²
› Gel strength (1.5%)―― >3,500 g/cm²
SeaKem® Gold Agarose产品应用:
• Preparative DNA and RNA electrophoresis/电泳制备DNA/RNA
• Analysis of Long PCR† reactions/长PCR反应分析
• Separation and further manipulation of DNA >1,000 bp/分离1000-50000 bp DNA
• Analytical electrophoresis of DNA and RNA /DNA和RNA分析电泳
• Blotting of DNA and RNA /DNA和RNA杂交
SeaKem® Gold Agarose 英文简述:
SeaKem® Gold Agarose is a very high gel strength, low EEO, standard gelling temperature agarose. This Genetic Technology Grade™ Agarose is for rapid resolution of megabase DNA by pulsed field gel electrophoresis (PFGE). Due to its low EEO, the electrophoretic mobility of DNA in SeaKem® Gold Agarose gels is significantly greater than in conventional agarose gels. Run times for PFGE can be decreased by as much as 50% depending upon buffer and agarose concentration. Due to its high gel strength, SeaKem® Gold Agarose forms easy-to-handle gels at low concentrations (as low as 0.5%), which allow for the separation of larger DNA fragments by conventional electrophoresis as well as a decrease in the time needed to separate DNA by PFGE. For the recovery of megabase size DNA fragments following PFGE separation, we recommend SeaPlaque™ GTG™ Agarose and ß-Agarase.
Related Products:
• AccuGENE® 10X TAE Buffer
• GelStar® Nucleic Acid Gel Stain
• Megabase DNA Standards
• InCert® Agarose
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文献和实验SDS agarose gels for analysis of proteins
. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose, SeaKem Gold (FMC BioProducts). TBE containing sodium dodecyl sulfate (SDS
Denaturing Agarose Gel Electrophoresis of RNA
under analysis. RNA molecular weight markers, an RNA sample known to be intact, or both, can be used for this purpose. Ambion''s NorthernMax® reagents for Northern Blotting include everything needed for denaturing agarose gel electrophoresis. These products
Recovering DNA from agarose gelsPaul N. Hengen, Ph.D. (July 14, 1999)IntroductionMethods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the internet. A commonly
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