抗促肾上腺皮质激素释放因子抗体

• Product nameAnti-Corticotropin Releasing Factor抗体
  参阅全部 Corticotropin Releasing Factor 一抗
• Description
  兔多克隆抗体to Corticotropin Releasing Factor
• Tested applicationsICC, RIA, WB, Nucleotide Array, IHC-Pmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
• Immunogen

  Synthetic peptide, corresponding to amino acids 1-41 of Rat Corticotropin Releasing Factor.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage bufferLiquid antiserum
• PurityWhole antiserum
• ClonalityPolyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurotransmitter
  • Neuropeptides
  • Hormones

  • Signal Transduction
  • Growth Factors/Hormones
  • Hormones

  • Neuroscience
  • Endocrine system
  • Hypothalamic pituitary adrenal axis

Applications

Target

• RelevanceCRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
• Cellular localizationCytoplasmic and Secreted
• Database links
  • Entrez Gene: 1392 Human
  • Entrez Gene: 12918 Mouse
  • Entrez Gene: 81648 Rat
  • Omim: 122560 Human
  • SwissProt: P06850 Human
  • SwissProt: Q8CIT0 Mouse
  • SwissProt: P01143 Rat
  • Unigene: 75294 Human

  see all
• Alternative names
  • Corticoliberin antibody
  • Corticoliberin precursor antibody
  • Corticotropin releasing factor antibody

  see all

Anti-Corticotropin Releasing Factor antibody images

• 

  Western blot - Anti-Corticotropin Releasing Factor antibody (ab8901)This image is courtesy of an Abreview submitted by Jean-Christophe Helbling
  Anti-Corticotropin Releasing Factor antibody (ab8901) at 1/2500 dilution + Mouse hypothalamus whole tissue lysate at 50 µg
  
  Secondary
  HRP-conjugated Donkey anti-rabbit IgG polyclonal - undiluted
  developed using the ECL technique
  
  Performed under non-reducing conditions.
  
  Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 17 kDa (possible non-specific binding),23 kDa (possible non-specific binding),35 kDa (possible non-specific binding).
  
  Exposure time : 1 minute

  This image is courtesy of an Abreview submitted by Jean-Christophe Helbling

  See Abreview

• 

  Radioimmunoassay - Anti-Corticotropin Releasing Factor antibody (ab8901)This image is a courtesy of an Anonymous Abreview
  Radioimmunoassay using ab8901
  - Day 1: Add Recombinant Protein A/G (10ug/ml) to a 96 wells plate; parafilm wrap; incubate overnight at 4°C.
  - Day 2: Aspirate and wash the plate to remove loosely bound Protein A/G; add antibody solution (dilution 1:50000); parafilm wrap; incubate 24h at 4°C.
  - Day 3: Aspirate and wash the plate to remove loosely bound antibody; add CRF standard (0, 0.03, 0.1, 0.3, 1, 3, 10, 30 ng/ml); parafilm wrap; incubate 24h at 4°C.
  - Day 4: DO NOT ASPIRATE; add ¹²⁵I-CRF; incubate 24h at 4°C.
  - Day 5: Aspirate and wash the plate; break the plate and collect the wells in scintillation tubes; add 2ml of scintillation liquid; count in beta counter (open the 3H window to at least 30-40 keV), at least 2min/tube.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corticotropin Releasing Factor antibody (ab8901)This image is courtesy of an anonymous Abreview
  Immunohistochemical analysis of rat brain tissue, staining Corticotropin Releasing Factor with ab8901.
  
  Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. The figure on the left is a secondary only control.

  See Abreview